Purdue/1 July 2008

Using 2 spots of filter paper to try to get more DNA
Using 16uL of TE instead of 10 (because the filter paper absorbs so much)
After spinning it down at 15000xg for 3 min., we're letting it sit out overnight to try to get more DNA
Tomorrow, we'll test how much DNA we have with a NanoDrop (more accurate and easy to use than the UV-Vis machine from earlier)
We'll also test the transformation efficiency with the Invitrogen kit that comes with the cells (pUC17 stuff)
Try to transform 2 parts:
- J09855 from 2008: it's from a DH5a strain, QC is great, and is on pSB1A2 plasmid
- Part from last year to compare filter paper w/ non-paper: J13003--it's a long part (like LacZ), on pSB1A2, and QC is great

Edited by Janie Stine