Purdue/25 September 2008

From 2008.igem.org

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Performed ligation reaction with LacZ and SOS:

  • Mixture:
    • 1uL T4 ligase
    • 2uL T4 buffer
    • 5uL LacZ DNA
    • 12uL SOS DNA
  • Let sit for 40 minutes at room temperature (should have been 10, but had to wait for the water bath to heat up)
  • Heat inactivated enzyme for 20 min at 55C
  • Craig, involved in learning Matlab, forgot about the enzyme. The water bath was at 58C when he took the enzyme out at 2:45PM. Heat inactivation for 1 hour, 15 minutes.

Edited by Janie Stine