Purdue/7 August 2008

From 2008.igem.org

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Jess digested o/n the mCherry parts to see if they're there...

  • 10uL DNA
  • 2uL Buffer #2
  • 8uL Mix (recipe below for 2 tubes)
    • 13uL ddH2O
    • 1uL EcoRI
    • 1uL SpeI
    • 1uL BSA
  • Put at 37C o/n
  • Ran on gel w/ Fast Blast
  • Found out that Fast Blast makes DNA Positive! (didn't work...)

Edited by Janie Stine