"
Rensselaer/8 September 2008
From 2008.igem.org
DNA purification digest
Fe(13ug) Plasmid(11ug)
DNA 280ul 95ul
XbaI 10 10
SprI 20 20
Buffer2 40 17.5
BSA 4 1.75
dH2O 46 30.75
____ _____
400 ul 175 ul
Insert checks digest
Fe-mRFP (~1ug)
DNA(30ug/ul) 33
MsCI(3U/ul) 6.7
EcoRI(2U/ul) 1
EcoBuff 5
BSA 0.5
dH2O 3.8
_____
50 ul
