User contributions
From 2008.igem.org
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- 23:11, 29 October 2008 (diff | hist) Team:Melbourne/Team
- 23:11, 29 October 2008 (diff | hist) Team:Melbourne/Team
- 23:11, 29 October 2008 (diff | hist) N File:MelbourneTeam.jpg (top)
- 14:27, 29 October 2008 (diff | hist) Team:Melbourne/Components (top)
- 14:27, 29 October 2008 (diff | hist) Template:MelbournePanel (top)
- 14:17, 29 October 2008 (diff | hist) Team:Melbourne/LinModel (top)
- 14:17, 29 October 2008 (diff | hist) Team:Melbourne/BinModel (top)
- 14:16, 29 October 2008 (diff | hist) Team:Melbourne/LabNote (top)
- 14:16, 29 October 2008 (diff | hist) Team:Melbourne/Products (top)
- 14:16, 29 October 2008 (diff | hist) Team:Melbourne/Components
- 14:16, 29 October 2008 (diff | hist) Team:Melbourne/Overview (top)
- 14:15, 29 October 2008 (diff | hist) Team:Melbourne/Team
- 14:15, 29 October 2008 (diff | hist) Team:Melbourne (top)
- 14:12, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 14:06, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 14:03, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 14:02, 29 October 2008 (diff | hist) N File:BinaryClock.pdf (top)
- 13:55, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 13:53, 29 October 2008 (diff | hist) Team:Melbourne/LinModel
- 13:53, 29 October 2008 (diff | hist) Team:Melbourne/BinModel
- 13:52, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 13:51, 29 October 2008 (diff | hist) Team:Melbourne/Team
- 13:51, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 13:51, 29 October 2008 (diff | hist) Team:Melbourne/Components
- 13:50, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 13:49, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 13:48, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 13:47, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 12:56, 29 October 2008 (diff | hist) Team:Melbourne/Overview
- 12:55, 29 October 2008 (diff | hist) Team:Melbourne/LabNote
- 12:55, 29 October 2008 (diff | hist) N Melbourne/5 August 2008 (New page: ==Done today:== * Red light retest. 4 transformations: *Cph8 + pPL-PCB *Cph8 *PCB *-ve control Protocol: *2μL DNA *RU1012 *30 min ice *1 min 42°C *1 hour at 37°C + LB Each transfor...) (top)
- 12:54, 29 October 2008 (diff | hist) N Melbourne/3 August 2008 (New page: ==Done today:== * Inoculated cultures with colonies from yesterday's transformations.) (top)
- 12:54, 29 October 2008 (diff | hist) N Melbourne/2 August 2008 (New page: ==Done today:== * Transformed the 3 ligations set up yesterday into DH5alpha cells. Plated on Amp.) (top)
- 12:54, 29 October 2008 (diff | hist) N Melbourne/1 August 2008 (New page: ==Done today:== * 3 Ligations left for overnight incubation: **pGlO with lock 3d (P2 8H) **pGLO with lock 3b (P2 8F) **pGLO with P8 1C (empty vector)) (top)
- 12:52, 29 October 2008 (diff | hist) N Melbourne/31 July 2008 (New page: ==Done today:== * Purified PCR products of pGLO (from mp 2, 3, 4) * Restriction digests left for overnight incubation: ** pGLO with X, P ** P8 1C with S, P ** P2 8F (lock 3b) with S, P **...) (top)
- 12:51, 29 October 2008 (diff | hist) N Melbourne/25 July 2008 (New page: ==Done today:== * Miniprepped: ** pGLO (again) as backup ** P0 3A ** P0 5A ** P14 8E ** P8 1C) (top)
- 12:51, 29 October 2008 (diff | hist) N Melbourne/24 July 2008 (New page: ==Done today:== * No growth from P13 7F or P3 4A from yesterday * Cultured successful transformations in appropriate antibiotic) (top)
- 12:51, 29 October 2008 (diff | hist) N Melbourne/23 July 2008 (New page: ==Done today:== * Transformed ** P13 7F (FBL) ** P14 8E (FBL) ** P0 5A (FBL) ** P0 3A (FBL) ** P3 4A (RS) See Biobrick section for w...) (top)
- 12:50, 29 October 2008 (diff | hist) N Melbourne/22 July 2008 (New page: ==Done today:== * Suspended and transformed P8 1C & P3 4A (empty Amp vectors). * Miniprepped pGLO.) (top)
- 12:49, 29 October 2008 (diff | hist) N Melbourne/18 July 2008 (New page: ==Done today:== * Plated the red light double transformation. Left to incubate on lab bench--one wrapped in foil, the other in glad wrap.) (top)
- 12:48, 29 October 2008 (diff | hist) N Melbourne/17 July 2008 (New page: ==Done today:== * Re-transformed P22 6E (RL), P13 4F, 8A (FBL)--plated on Kan+IPTG * Double transformation of pLPCB & CPH8 into RU1012 cells--to be plated tomorrow on Amp+Kan+Chlor+IPTG+X-...) (top)
- 12:48, 29 October 2008 (diff | hist) N Melbourne/15 July 2008 (New page: ==Done today:== * Miniprepped PO 3F, 3E, 4C, 4G * Set up cultures P1 7E, P13 9G) (top)
- 12:48, 29 October 2008 (diff | hist) N Melbourne/14 July 2008 (New page: ==Done today:== * Re-transformed: ** P13 4F, 8A (FBL) ** P22 6E, P13 9G (RL) ** P1 7E (RS) ''Result:'' Only P13 9G and P1 7E grew. * Made Kan plates) (top)
- 12:47, 29 October 2008 (diff | hist) N Melbourne/11 July 2008 (New page: ==Done today:== * Miniprepped P13 9H, P6 3D, P2 9A, P13 5E, P13 5C) (top)
- 12:46, 29 October 2008 (diff | hist) N Melbourne/9 July 2008 (New page: ==Done today:== * Re-transformed P13 9H (RL), P6 3D (RS) * Suspended and transformed P2 9A (RS) Used slightly modified protocol. All plates showed growth.) (top)
- 12:46, 29 October 2008 (diff | hist) N Melbourne/8 July 2008 (New page: ==Done today:== * Retransformed : P13 4F, 8A (FBL) P13 9G, P13 9H, P22 6E (RL) Also set up a positive control (pET 30-8)--Kanamycin resistant vector * Suspended and transformed P6 3...) (top)
- 12:45, 29 October 2008 (diff | hist) N Melbourne/3 July 2008 (New page: ==Done today:== * Miniprepped: ** P0 C4, E3, F3, G4 (FBL) ** P3 8C, P1 12F (RS) * Made glycerol stocks * Transformed: ** P22 8C, 6E ** P16 5F ** P13 5E, 9G ** P2 5E ** P0 2E * Punched:...) (top)
- 12:44, 29 October 2008 (diff | hist) N Melbourne/1 July 2008 (New page: ==Done today:== * Suspended from Kit {| border = "0.5" |- ! Part ! Plate ! Well |- | M30109 | 1022 | 8C |- | I15010 | 1022 | 6E |- | I15008 | 1013 | 9G |- | I15009 | 1013 | 5E |- | R00...) (top)
- 12:43, 29 October 2008 (diff | hist) N Melbourne/30 June 2008 (New page: ==Done today:== * Made competent DH5alpha cells.) (top)
- 12:43, 29 October 2008 (diff | hist) N Melbourne/1 June 2008 (New page: ==Done today:== * Suspension and transformation (of DH5alpha cells) with P1003 8C (J36846 pTet mRFP)...for ribolock as reporter P1002 8A (I13522 pTet GFP) P1002 3H (I13600 pTet CFP) ...) (top)
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