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User contributions
From 2008.igem.org
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- 21:37, 11 October 2008 (diff | hist) Jamboree/Schedule/Practice sessions (→Friday November 7 : Practice Talks sign-up sheet)
- 16:42, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 27, 2008) (top)
- 16:41, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 26, 2008)
- 16:32, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 26, 2008)
- 16:30, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 26, 2008)
- 16:29, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 26)
- 16:28, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 25)
- 16:27, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 26)
- 16:25, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 26)
- 16:24, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 22, 2008)
- 16:23, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 20,2008)
- 16:22, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 11, 2008)
- 16:22, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 8, 2008)
- 16:21, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 7,2008)
- 16:20, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→August 6, 2008)
- 16:18, 28 September 2008 (diff | hist) Team:University of Chicago/Notebook/Norayucel (→July 29, 2008)
- 15:15, 8 August 2008 (diff | hist) Team:University of Chicago/Notebook/Transformations (→iGEM transformatin (using paper spots)) (top)
- 15:15, 8 August 2008 (diff | hist) Team:University of Chicago/Notebook/Transformations (→iGEM transformatin (using paper spots))
- 15:15, 8 August 2008 (diff | hist) Team:University of Chicago/Notebook/Transformations (→iGEM transformatin (using paper spots))
- 15:14, 8 August 2008 (diff | hist) Team:University of Chicago/Notebook/Transformations (→General transformation)
- 15:05, 8 August 2008 (diff | hist) Team:University of Chicago/Notebook/Transformations (→=General transformation)
- 15:05, 8 August 2008 (diff | hist) Team:University of Chicago/Notebook/Transformations (→=General transformation)
- 15:04, 8 August 2008 (diff | hist) Team:University of Chicago/Notebook/Transformations (→=General transformation)
- 15:03, 8 August 2008 (diff | hist) N Team:University of Chicago/Notebook/Transformations (New page: ==Chemocompetent cells== ===General transformation== #Transform 50 _l of cells with 1 _l of standard pUC19 plasmid (Invitrogen) * This is at 10 pg/_l or 10-5 _g/_l * This can be made by ...)
- 15:00, 8 August 2008 (diff | hist) Team:University of Chicago/Notebook (→Protocols)
- 16:18, 7 August 2008 (diff | hist) Team:University of Chicago/Notebook/DH5-alpha competent cells (→Procedure) (top)
- 16:17, 7 August 2008 (diff | hist) N Team:University of Chicago/Notebook/DH5-alpha competent cells (New page: ==Materials== ===TB Solution=== *10mM PIPES *15mM CaCl2 *250mM KCl *Dissolve in nanopure water and adjust pH 6.7 with KOH or HCL (solutes will dissolve as you do this) and then add 55mM M...)
- 15:53, 7 August 2008 (diff | hist) Team:University of Chicago/Notebook (→Protocols)
- 19:14, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook/Plasmids (→pBluescript SK) (top)
- 19:13, 29 July 2008 (diff | hist) N Team:University of Chicago/Notebook/Plasmids (New page: ==pBluescript SK== [http://www.addgene.org/pgvec1?f=v&cmd=showvecinfo&vectorid=5495 Plasmid map source information])
- 19:09, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook (→Materials)
- 19:02, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook (→Recipes)
- 18:56, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook/DNA mini-prep (→Zyppy mini-prep) (top)
- 18:55, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook/DNA mini-prep (→Zyppy mini-prep)
- 18:54, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook/DNA mini-prep (→Zyppy mini-prep)
- 18:54, 29 July 2008 (diff | hist) N Team:University of Chicago/Notebook/DNA mini-prep (New page: ====Zyppy mini-prep==== The following procedure is performed at room temperature. Ensure that RNase A has been added to the Neutralization Buffer, ethanol has been added to the Zyppy™...)
- 18:53, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook (→DNA prep)
- 18:50, 29 July 2008 (diff | hist) N Team:University of Chicago/Notebook/Glycerol stocks (New page: ===== Glycerol Stocks ===== ====== Materials ====== * 40% glycerol solution * Cryogenic vials ====== Method ====== # Add 1 ml of 40% glycerol in H2O to a cryogenic vial. # Add 1 ml sa...) (top)
- 18:50, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook (→Protocols)
- 18:47, 29 July 2008 (diff | hist) N Team:University of Chicago/Notebook/TOP10 competent cells (New page: ==== TOP10 Competent Cells ==== * Prechill plasticware and glassware ===== Preparing seed stocks ===== # Streak TOP10 cells on an SOB plate and grow for single colonies at 23°C #* room ...) (top)
- 18:46, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook (→Protocols)
- 18:44, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook (→Recipes)
- 18:43, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook/Gels (→1.2%) (top)
- 18:43, 29 July 2008 (diff | hist) N Team:University of Chicago/Notebook/Gels (New page: === DNA and protein gels === ==== Agar ==== ===== 0.8% ===== # Weigh out .8g agarose # Put agarose in 500mL Erlenmeyer flask. # Add 100mL 1X TBE Buffer. Swirl. # Microwave at 100% power un...)
- 18:43, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook (→DNA and protein gels)
- 18:43, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook (→Recipes)
- 18:41, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook (→Media)
- 18:41, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook (→Buffers)
- 18:40, 29 July 2008 (diff | hist) N Team:University of Chicago/Notebook/Buffers (New page: === Buffers === ==== CCMB80 ==== * 10 mM KOAc pH 7.0 (10 ml of a 1M stock/L) * 80 mM CaCl2.2H2O (11.8 g/L) * 20 mM MnCl2.4H2O (4.0 g/L) * 10 mM MgCl2.6H2O (2.0 g/L) * 10% glycerol (10...)
- 18:39, 29 July 2008 (diff | hist) Team:University of Chicago/Notebook (→Media)
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