User contributions
From 2008.igem.org
- 13:08, 8 September 2008 (diff | hist) Rensselaer/4 September 2008 (top)
- 21:06, 4 September 2008 (diff | hist) N Rensselaer/4 September 2008 (New page: '''Promoter Group''' ''' Gel Run''')
- 21:06, 4 September 2008 (diff | hist) N Rensselaer/26 August 2008 (New page: '''Promoter Group''' Spin restriction digests down. Store @ -20C) (top)
- 21:05, 4 September 2008 (diff | hist) N Rensselaer/25 August 2008 (New page: '''Promoter Group''' '''Restriction Digest of Ligation Colonies Expressing RFP''' H2O 31ul 31ul Buffer 4 4ul 4ul DNA: Ligation DNA...) (top)
- 21:01, 4 September 2008 (diff | hist) N Rensselaer/24 August 2008 (New page: Promoter Group '''Quick Screen of Fe Promoter Ligation Colony and J12006 Plasmid Culture''' One of the j12006 cultures was not red (must have lost plasmid). quick screen only one j12006...) (top)
- 18:27, 23 August 2008 (diff | hist) N Rensselaer/23 August 2008 (New page: '''Promoter Group''' '''Aplification of J61002 mRFP plasmid''' Pick 2 colonies to 2 15 ml tubes with 5 mls 2XYT and 75 ug/ml ampicillin. Grow @ 37C ON w/ shaking. ''' '''Fe Promoter Li...) (top)
- 21:30, 10 August 2008 (diff | hist) Rensselaer/10 August 2008 (top)
- 21:00, 10 August 2008 (diff | hist) Rensselaer/10 August 2008
- 20:32, 10 August 2008 (diff | hist) N Rensselaer/10 August 2008 (New page: '''Promoter Group''' '''Miniprep of Ligation Transformed Cultures using Promega Wizard Plus Miniprep kit.''' ''Production of a Cleared Lysate'' Harvest cells by spinning @ 4000 RPMs, 10...)
- 19:12, 9 August 2008 (diff | hist) N Rensselaer/9 August 2008 (New page: '''Promoter Group''' Concentrated plate is confluent. Undiluted plate is semi confluent. Pick 10 single colonies from undiluted plate to 5 mls 2XYT + 75 ug/ml ampicillin. Incubate @ 37C...) (top)
- 21:20, 8 August 2008 (diff | hist) N Rensselaer/8 August 2008 (New page: '''Promoter Group''' '''Transformation of Ligation ''' Thaw TOP10 supercompetent cells on ice. Add 5 ul of Fe/mRFP ligation. Tap tube to mix. Incubate on ice 30'. Heat shock cells @ 4...) (top)
- 19:56, 7 August 2008 (diff | hist) Rensselaer/7 August 2008 (top)
- 18:07, 7 August 2008 (diff | hist) Rensselaer/7 August 2008
- 18:03, 7 August 2008 (diff | hist) Rensselaer/7 August 2008
- 18:01, 7 August 2008 (diff | hist) Rensselaer/7 August 2008
- 17:55, 7 August 2008 (diff | hist) Rensselaer/7 August 2008
- 15:46, 7 August 2008 (diff | hist) Rensselaer/7 August 2008
- 15:46, 7 August 2008 (diff | hist) N Rensselaer/7 August 2008 (New page: '''Promoter Group''' '''Ligation of Fe promoter in mRFP plasmid''' j61002 = 3002 bp Fe promoter insert = 1044 bp Fe promoter vector (j61002 - tet - mRFP) = 2252 bp mRFP vector (j61002 - ...)
- 15:24, 7 August 2008 (diff | hist) N Rensselaer/6 August 2008 (New page: '''Promoter Group''' '''Buffer exchange/purification of XbaI/SpeI digests of mRFP and Fe promoter plasmids using Promega Wizard SV Gel and PCR Clean-Up System''' Add 40 ul (mRFP digest)/...) (top)
- 15:15, 7 August 2008 (diff | hist) N Rensselaer/5 August 2008 (New page: '''Promoter Group''' Set up XbaI/SpeI restriction digest of mRFP and Fe promoter plasmids. Digest 2 ug of each plasmid, let reaction go @ 37C ON.) (top)
- 19:24, 4 August 2008 (diff | hist) N Rensselaer/1 August 2008 (New page: '''MiniPrep of Fe Promoter Transformed Colonies''') (top)
- 19:23, 4 August 2008 (diff | hist) Rensselaer/31 July 2008 (top)
- 19:19, 4 August 2008 (diff | hist) N Rensselaer/31 July 2008 (New page: '''Results of Transformation''' Three colonies: 2 on undiluted plate and 1 on 50% concentrated plate Pick all three colonies into 5 mls 2XYT + 100 ug/ml ampicillin. Grow overnight @ 37C...)
- 14:20, 30 July 2008 (diff | hist) N Rensselaer/30 July 2008 (New page: Promoter Group - '''7.30.08''' '''Transformation of Fe Promoter''' (Plate 1007 Well 7C) Into TOP10 ''E. coli'' Set up petri dishes with bleach, dH2O, ethanol for wash of punch tool. Ali...)
- 23:09, 28 July 2008 (diff | hist) Team:Rensselaer/Project (→Overall project) (top)
- 23:09, 28 July 2008 (diff | hist) Team:Rensselaer/Project (→Overall project)