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Measuring Minimum Inhibitory Concentration
- Grow one 5 ml culture to saturation at 37C with shaking in the indicated media and antibiotics as necessary.
- Back dilute parent culture 1:50 into 5 ml of the same media and antibiotics plus differing amounts of hydrogen peroxide and let shake at 37C overnight.
- Record MIC as the concentration of hydrogen peroxide necessary to prevent overnight turbid growth.
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