Team:Chiba/Calendar-Home/18 August 2008

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17 August 2008 <|> 19 August 2008

Contents

Laboratory work

Team:Input

Transformation

  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2006)
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416](2007)

competent cells : XL10Gold(Cm),LB-agar(KanR)

Team:communication

PCR
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2008)
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2008)
  • [http://partsregistry.org/Part:BBa_C0077 BBa_C0077](2008)
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2008)
  • [http://partsregistry.org/Part:BBa_C0079 BBa_C0079](2008)
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control


DNA Template(μL) 1
dNTP mix(μL) 10
Foward Primer(μL) 5
Reverse Primer(μL) 5
DNA polymerase(μL) 1
Thermopol Buffer(μL) 10
dH2O(μL) 68
TOTAL(μL) 100


95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C


--->Gel Check


Chiba-0818.JPG
Sample DNA 3
Loading Dye(μL) 2
dH2O(μL) 7
TOTAL(μL) 12
From left;
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2008) ->None
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2008) ->None
  • [http://partsregistry.org/Part:BBa_C0077 BBa_C0077](2008) ->None
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2008) ->None
  • [http://partsregistry.org/Part:BBa_C0079 BBa_C0079](2008) ->None
  • [http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)･･･control ->OK



Transformation

competent cells : XL10G

  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2007)
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070](2006)
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2007)
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076](2006) -> No colonies on plates(19/8)
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2007)
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078](2006)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2007)
  • [http://partsregistry.org/Part:BBa_C0170 BBa_C0170](2006)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2007)
  • [http://partsregistry.org/Part:BBa_C0178 BBa_C0178](2006)


-->(20/8)Mini prep

Team:Output

no work

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