Team:Chiba/Calendar-Home/20 August 2008

From 2008.igem.org

>Home | Notebook

19 August 2008 <|> 21 August 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

PCR

BBa_J22136(2007)(1)
BBa_J22136(2008)(2)
BBa_J22141(2007)(3)
BBa_J22141(2008)(4)
BBa_E0612(2008)(5)

Sample No.	1	2	3	4	5
DNA tamplate(μL)	1	1	1	1	1
FW primer(μL)	5	5	5	5	5
VR primer(μL)	5	5	5	5	5
dNTPmix(μL)	10	10	10	10	10
thermo pol buffer(μL)	10	10	10	10	10
DNA pol(VENT)(μL)	1	1	1	1	1
dH₂O(μL)	68	68	68	68	68
TOTAL(μL)	100	100	100	100	100

-->Gel Check

Sample No.	1	2	3	4	5
Sample DNA	1	1	1	1	1
loading dye(μL)	1	1	1	1	1
dH₂O(μL)	4	4	4	4	4
Total(μL)	6	6	6	6	6

-->1,2,3,4,5:none

Transformation

BBa_R0051(2007)
BBa_R0051(2006)
BBa_J06650(2007)
BBa_J06650(2006)
BBa_J22136(2007)
BBa_J22141(2007)-->only one colony

Team:Communication

(18/8)--->Mini prep

BBa_C0070(2007)
BBa_C0070(2006)
BBa_C0076(2007)
BBa_C0078(2007)
BBa_C0078(2006)
BBa_C0170(2007)
BBa_C0170(2006)
BBa_C0178(2007)
BBa_C0178(2006)

--->Gel Check

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

BBa_C0076(2007) -> None
BBa_C0078(2007) -> None
BBa_C0078(2006) -> None

Sample DNA	10
Loading Dye	4
dH₂O	10
TOTAL	24μL

From left;

BBa_C0078(2007) -> None
BBa_C0078(2006) -> OK
BBa_C0076(2007) -> None
BBa_C0070(2007) -> OK
BBa_C0070(2006) -> OK

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

BBa_C0170(2007) -> OK
BBa_C0170(2006) -> OK
BBa_C0178(2007) -> OK
BBa_C0178(2006) -> OK

--->Digestion test

BBa_C0170(2007)
BBa_C0170(2006)
BBa_C0178(2007)
BBa_C0178(2006)

Sample No	1	2	3	4	5	6	7	8
BBa_C0170(2006)	3	-	-	-	3	-	-	-
BBa_C0170(2007)	-	3	-	-	-	3	-	-
BBa_C0178(2006)	-	-	3	-	-	-	3	-
BBa_C0178(2007)	-	-	-	3	-	-	-	3
EcoRI	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1
PstI	-	-	-	-	0.1	0.1	0.1	0.1
Buffer EcoRI	1	1	1	1	1	1	1	1
BSA	1	1	1	1	1	1	1	1
dH₂O	4.9	4.9	4.9	4.9	4.8	4.8	4.8	4.8
TOTAL	10μL	10μL	10μL	10μL	10μL	10μL	10μL	10μL

--->Gel Check

Sample DNA	3
Loading Dye	2
dH₂O	7
TOTAL	12μL

From left;

(Single Digestion 1~4)

BBa_C0170(2006) -> OK
BBa_C0170(2007) -> OK
BBa_C0178(2006) -> OK
BBa_C0178(2007) -> OK

(Double Digestion 1~4)

BBa_C0170(2006) -> OK
BBa_C0170(2007) -> OK
BBa_C0178(2006) -> OK
BBa_C0178(2007) -> OK

Team:Output

BBa_J52008(2007)-->colony
BBa_I712019(2008)-->no colony
BBa_J33202(2007)-->colony
BBa_E2030(2007)-->not so good
YFP(P-GEX)-->colony(Amp)
BBa_F2620(2007)-->colony
BBa_T9002(2007)-->colony

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/20_August_2008"