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Team:Chiba/Calendar-Home/23 September 2008
From 2008.igem.org
22 September 2008 <|> 24 September 2008
Laboratory work
Team:Communication
- time-delay test
- transformants
- [http://partsregistry.org/Part:BBa_S03623 BBa_S03623]
- [http://partsregistry.org/Part:BBa_K084007 BBa_K084007]
- [http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
- [http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084012 BBa_K084012]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K084014 BBa_K084014]
- E.coli strain
Senders:XL10GOLD
Receiver:JW1908 - reaction temparature
37°C and 30°C
- transformants
- Result(at 37°C)
- Receiver cells/Sender cells = 1
File:Chiba communication 0923 01.xls
- Receiver cells/Sender cells = 10
- Result(at 30°C)
- Receiver cells/Sender cells = 1
- Receiver cells/Sender cells = 10
Team:Output
Digestion test
[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]
| DNA(μL) | 2 |
| EcoRI(μL) | 0.5 |
| PstI(μL) | 0.5 |
| BSA(×10) | 1 |
| EcoRI Buffer(&time;10) | 1 |
| dH2O(μL) | 5 |
| TOTAL(μL) | 10 |
TRANSFORMATION
[http://partsregistry.org/Part:BBa_I6107 BBa_I6107]
[http://partsregistry.org/Part:BBa_I6206 BBa_I6206]
[http://partsregistry.org/Part:BBa_I6101 BBa_I6101]
[http://partsregistry.org/Part:BBa_I6042 BBa_I6042]
[http://partsregistry.org/Part:BBa_I0462 BBa_I0462]
Digestion
Vector:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]:1
Insert:[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]:2
Vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]:3
Insert:[http://partsregistry.org/Part:BBa_R0062 BBa_R0062]:4
Insert:[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]:5
| Sample No | 1 | 2 | 3 | 4 | 5 |
| DNA(μL) | 25 | 25 | 25 | 25 | 25 |
| EcoRI(μL) | - | - | 1 | 1 | - |
| SpeI(μL) | 1 | - | 1 | 1 | - |
| PstI(μL) | 1 | 1 | 1 | - | 1 |
| XbaI(μL) | - | 1 | - | - | 1 |
| BSA(×10)(μL) | 4 | 4 | 4 | - | 4 |
| EcoRI Buffer(&time;10)(μL) | - | - | 4 | 4 | - |
| Buffer 2(μL) | 4 | - | - | - | - |
| Buffer 3(μL) | - | 4 | - | - | 4 |
| dH2O(μL) | 5 | 5 | 5 | 9 | 5 |
| TOTAL(μL) | 40 | 40 | 40 | 40 | 40 |
->1 hour 37°C
->SAP 1μL(Vector only) ->1 hour 37°C ->30 minutes 65°C
->Zymo ->10μL
Gel check
| DNA(μL) | 1 |
| Loading Dye(μL) | 1 |
| dH2O(μL) | 4 |
| TOTAL(μL) | 6 |
->3&4&5Ligation:not yet
