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Team:Chiba/Calendar-Home/27 August 2008
From 2008.igem.org
26 August 2008 <|> 28 August 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- Sample DNA :BBa_R0010
| Sample No. | 1 | 2 | 3 |
| Sample DNA | 1 | 1 | 1 |
| PstI | - | - | 0.5 |
| EcoRI | - | 0.5 | 0.5 |
| Buffer 3 | - | 1 | 1 |
| BSA | - | - | 1 |
| dH2O | - | 7.5 | 2 |
| TOTAL | 1μl | 10μl | 10μl |
- --->Gel Check
|
|
- (26/8)--->Colony-PCR
- Colony PCR of 5 colonies from ligation plates (26/8-(4),(5)) and one from control plate(BBa_F2620(2007)).
DNA Template 1 dNTP mix 5 Foward Primer 5 Reverse Primer 5 DNA polymerase 0.5 Thermopol Buffer 5 dH2O 28.5 TOTAL 50μL
- 95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C
- --->Gel Check
Sample DNA 10 Loading Dye 2 TOTAL 12μl - From left;
- 1~5 insert:BBa_C0170 + vector:BBa_J04500 -> OK
- 6 BBa_F2620(2007)-->Positive Control -> OK
- 7~11 insert:BBa_C0178 + vector:BBa_J04500 -> OK
- From left;
- --->(28/8)Miniprep
- competent cells : XL10G
- BBa_C0062(2007)
- BBa_C0062(2006) -> no colony on plates
- BBa_C0179(2007)
- BBa_C0179(2006)
- BBa_S03156(2007)
- BBa_S03156(2006)
- BBa_S03158(2007)
- BBa_S03158(2006)
- BBa_S03160(2007)
- BBa_S0316(2006)
- --->Mini prep
- --->(29/8)Gel Check
Team:Output
no work
