Team:Chiba/parts/BBa K084007

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parts usage

BBa_K084007(plac+rbs+LasI(no LVA))

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日本語:IPTGにより、シュードモナス由来の3OC12HSL合成酵素,LasIを発現するデバイス。

English:

Experiment

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

  1. Transformed Senders into E.coli strains(XL10GOLD or JW1908) and Receiver into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37°C 12h
  3. Mixed them.
  4. Incubated at 30°C.
  5. Measured intensity of green fluorescence at regular time intervals.
  1. E.Coli strain JW1908 or XL10Gold.
  2. Reaction Temparature 37 or 30.
  3. Cell number.

Results

Sender:JW1908, Receiver:JW1908, 37°C

Fig.　　
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:JW1908,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.

Sender:JW1908, Receiver:JW1908, 30°C

Fig.　　
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,Senders:JW1908,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.

Senders:XL10Gold, Receiver:JW1908, 37°C

Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.

Senders:XL10Gold, Receiver:JW1908, 30°C

Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.


others

Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Fig.　　
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30,Receiver cells/Sender cells = 1.
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