Team:Chiba/protocol/DNA Purification/zymocleam

From 2008.igem.org

DNA Cleam&Concentrator Kit

  • Add 2 volume of DNA binding buffer to each volume of DNA sample, Use voltex to mix.
  • Load mixture silica column and place column into a 2 ml collection tube
  • Centrifuge at full speed for 30 sec. Discard the flow-through.
  • Add 200μL of wash buffer and spin 30 sec.
  • Place siliva volumn into a new 1.5 ml tube. Add water directly to the column matrix and spin to elute the DNA.