Team:ESBS-Strasbourg/8 October 2008

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Contents

DryLab

Modeling

WetLab

Katja:

results of the last two days:
We have

  • mCherry_L_Vp16_L_lexA_L
  • CFP_cin8_adh1term (Manuels work)
  • Gal1_CFP_NLS_adh1term
  • Gal1_CFP_hsl1_adhterm
  • Gal1_mCherrry_L_lexA_L_VP16_L


So further assemblies will be:

  • Gal1 (BV - in the freezer) + mCherry_L_Vp16_L_lexA_L (BI - to be done)
  • Gal1 (BV - in the freezer) + lexA_L_Vp16_L_mCherry_L (BI - to be done)
  • Gal1 (BV - in the freezer) + CFP_cln2_adhterm (BI - to be remake)
  • Gal1 (BV - in the freezer) + CFP_cin8-adhterm (BI - to be done)
  • Gal1_mCherry_L_lexA_L_Vp16_L (FI - to be done) + NLS_adhterm (FV - to be done)
  • Gal1_mCherry_L_lexA_L_Vp16_L (BV - to be done) + NLS_adhterm (BI - to be done) (for savety)
  • Leu2 (BI) in pSB1A2 (X+P)
  • Ura3 (FI) in pSB1A2 (E+S)


General

Katja:
I ordered primers for the Mutagenesis of Leu2 (silent mutation) and Ura3 (in non coding region) so that we can deleted the EcoRI and Pst1 site respectively. According to Mr. Chatton there shouldn't be a problem with transcriptional/translational regulation at this point.

Quote of the day

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