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Team:Edinburgh/ISO2
From 2008.igem.org
Contents |
ISO2
Amino Acid Sequence
MASSLPAPPASPSSSWRGLTPRCPPPRCGPLLARAAARSYRYRFRTDDDG
VVDVAVAGKDGDAGYVVAIEAPTHGQRGGLVLRPAGSGEGVPLAPAAPGG
ALVAELSYDVARAPFHVSFTLADAMGAEIRTHRGTSFRVPVGVGRGCPSP
LGLSQSKDGAANFAVYSKIAKGMVLCLFGGGGGDGPALEIELDPYVHRTG
DVWHVSMESVEGYARYGFRSGLFAMFGIDRPLLDPYAKVIGDFVAGDSVD
EDGLAVPSIRCLASLKNAPNYDWGRDKHPCLPLQKLVVYRANVALFTKDR
SSRLADNAAGTFSGMSAKVEHFRHLGVNAVLLEPVFPFHQVKGPYFPYHF
FSPMSLYSSECSSVSAIKSMKDMVKTMHRNGIEVLLEVVFTHTAEGGAEC
QMISLRGIDGSSYYIADGIAGCKASVLNCNHPVTQKLILDSLRHWVLDFH
VDGFCFINAPFLVRGPGGEGLSRPPLLEAIAFDPVLSKTKIIADPWSPLD
ISNVQFPFPHWKRWAEMNTRFSMDVRKFLKGEALISDLATRLCGSGDLFS
SRGPAFSFNYVSRNSGLTLVDLVSFSSDELASEFSWNYGEEGPSENNAVF
QTRLRQIHNFLFILFISLGIPVLNMGDECGNSAAGSTSYKDRGLLNWKAL
KTAFVKEVTGFISFLSALRSRRADIFQRCEFLKLENIHWYGSDLSEPCWE
DPTSNFLCLHINAELDEKLPDSTGGDLYICFNANEESASATLPAIAEGSM
WLRLVDTSLAFPGFFSRGSSHETHQVLGFSSYQVKAHSCVLFESKRVLS*
DNA Sequence
atggcctcctccctccccgcgccgccggcctcgccctcttcctcctggcg 50
accggaggagggaggggcgcggcggccggagcgggagaaggaggaccgc
cggactcacgccccgctgccctccgcctcgctgcggtcccctcctcgccc 100
gcctgagtgcggggcgacgggaggcggagcgacgccaggggaggagcggg
gcgcggcagcgcgttcttaccgttaccgcttccgaaccgacgacgacggc 150
cgcgccgtcgcgcaagaatggcaatggcgaaggcttggctgctgctgccg
gtggtggacgtggccgtcgccgggaaagacggcgatgcggggtatgtggt 200
caccacctgcaccggcagcggccctttctgccgctacgccccatacacca
cgctatcgaggctcctacccatggacagaggggcggtcttgtgctccgcc 250
gcgatagctccgaggatgggtacctgtctccccgccagaacacgaggcgg
ccgccggctccggcgagggcgtccctctggccccagccgcgccgggaggt 300
ggcggccgaggccgctcccgcagggagaccggggtcggcgcggccctcca
gccctcgtggctgagttgtcctacgacgtggcccgcgcgccgttccacgt 350
cgggagcaccgactcaacaggatgctgcaccgggcgcgcggcaaggtgca
ctcgttcacgctggccgacgcgatgggagcggagatacggacacaccgcg 400
gagcaagtgcgaccggctgcgctaccctcgcctctatgcctgtgtggcgc
ggacgagcttccgcgtgcctgttggcgtcggacggggctgcccctcgccg 450
cctgctcgaaggcgcacggacaaccgcagcctgccccgacggggagcggc
ctcggcctgtcccagtccaaggatggggccgctaacttcgcggtttacag 500
gagccggacagggtcaggttcctaccccggcgattgaagcgccaaatgtc
caagatcgccaagggcatggtgctctgcctcttcggtggtggcggcgggg 550
gttctagcggttcccgtaccacgagacggagaagccaccaccgccgcccc
acggacccgcgcttgagattgagctcgacccgtacgtccaccggaccggc 600
tgcctgggcgcgaactctaactcgagctgggcatgcaggtggcctggccg
gatgtctggcacgtctcgatggagagcgtggaggggtacgcccgctacgg 650
ctacagaccgtgcagagctacctctcgcacctccccatgcgggcgatgcc
cttccgcagcgggctgttcgcaatgtttggcattgaccgcccgctactcg 700
gaaggcgtcgcccgacaagcgttacaaaccgtaactggcgggcgatgagc
acccgtacgccaaggtgatcggggacttcgtcgctggcgactctgttgat 750
tgggcatgcggttccactagcccctgaagcagcgaccgctgagacaacta
gaggatgggctagctgtgccatccataaggtgtctcgcgtccttgaagaa 800
ctcctacccgatcgacacggtaggtattccacagagcgcaggaacttctt
tgcacccaactacgattggggcagggacaagcacccatgcttgccattgc 850
acgtgggttgatgctaaccccgtccctgttcgtgggtacgaacggtaacg
agaagctggtggtctaccgggcaaatgtagctttgttcaccaaggatagg 900
tcttcgaccaccagatggcccgtttacatcgaaacaagtggttcctatcc
tcgagtcggctggcagacaatgccgctggtactttctccggcatgtctgc 950
agctcagccgaccgtctgttacggcgaccatgaaagaggccgtacagacg
aaaggtggaacacttcaggcatcttggtgtcaatgcagttttgctggagc 1000
tttccaccttgtgaagtccgtagaaccacagttacgtcaaaacgacctcg
cagttttcccattccaccaagtgaagggaccatattttccataccatttt 1050
gtcaaaagggtaaggtggttcacttccctggtataaaaggtatggtaaaa
ttttcacctatgagcttgtatagcagtgaatgctccagtgtttcagctat 1100
aaaagtggatactcgaacatatcgtcacttacgaggtcacaaagtcgata
caagtctatgaaggatatggtcaaaacaatgcacagaaatggaatagagg 1150
gttcagatacttcctataccagttttgttacgtgtctttaccttatctcc
ttctcttggaggttgttttcacgcatactgctgaaggaggggcggagtgt 1200
aagagaacctccaacaaaagtgcgtatgacgacttcctccccgcctcaca
cagatgatatcacttcgaggcatcgatggttcctcgtactacattgctga 1250
gtctactatagtgaagctccgtagctaccaaggagcatgatgtaacgact
tggaatcgctggatgcaaggcaagtgtgttgaattgcaaccatccagtga 1300
accttagcgacctacgttccgttcacacaacttaacgttggtaggtcact
ctcagaagctgattttggacagcctccgccattgggtgctcgacttccat 1350
gagtcttcgactaaaacctgtcggaggcggtaacccacgagctgaaggta
gttgatgggttctgcttcatcaatgctcctttcctcgtcagaggtccagg 1400
caactacccaagacgaagtagttacgaggaaaggagcagtctccaggtcc
tggtgagggcctctcacggcctccacttctggaagccatagcatttgatc 1450
accactcccggagagtgccggaggtgaagaccttcggtatcgtaaactag
ctgttctttcaaagactaagatcattgcagatccttggtctccgcttgac 1500
gacaagaaagtttctgattctagtaacgtctaggaaccagaggcgaactg
atatctaatgtgcaatttccattccctcattggaaaagatgggctgagat 1550
tatagattacacgttaaaggtaagggagtaaccttttctacccgactcta
gaacacaagattctctatggatgtgcgcaagtttcttaagggagaagcac 1600
cttgtgttctaagagatacctacacgcgttcaaagaattccctcttcgtg
ttatcagtgatcttgctacacgtttgtgtggcagtggggacttattttcc 1650
aatagtcactagaacgatgtgcaaacacaccgtcacccctgaataaaagg
tcaaggggcccagcattttcgttcaattatgtatccaggaattctggact 1700
agttccccgggtcgtaaaagcaagttaatacataggtccttaagacctga
cactcttgttgatctagtgagcttcagcagtgatgagcttgcttctgagt 1750
gtgagaacaactagatcactcgaagtcgtcactactcgaacgaagactca
tcagctggaattatggtgaagaaggaccatcggagaacaacgcagtcttt 1800
agtcgaccttaataccacttcttcctggtagcctcttgttgcgtcagaaa
caaaccaggctaagacagatacacaacttcctgtttattctattcatttc 1850
gtttggtccgattctgtctatgtgttgaaggacaaataagataagtaaag
ccttggtattcctgttcttaacatgggggatgaatgtggaaactcagctg 1900
ggaaccataaggacaagaattgtaccccctacttacacctttgagtcgac
ctggttcaacatcatacaaggatagagggcttctgaactggaaagccttg 1950
gaccaagttgtagtatgttcctatctcccgaagacttgacctttcggaac
aagaccgcttttgttaaggaagttaccgggtttatttcgtttctatctgc 2000
ttctggcgaaaacaattccttcaatggcccaaataaagcaaagatagacg
actaaggagtcgacgagcagacattttccagagatgcgagtttctaaaac 2050
tgattcctcagctgctcgtctgtaaaaggtctctacgctcaaagattttg
ttgaaaatatacattggtatgggagtgatttatctgagccatgttgggag 2100
aacttttatatgtaaccataccctcactaaatagactcggtacaaccctc
gatcctactagcaactttctttgcttgcacataaatgcagagctggacga 2150
ctaggatgatcgttgaaagaaacgaacgtgtatttacgtctcgacctgct
gaagctaccagattcgactggaggtgatttgtatatctgtttcaatgcaa 2200
cttcgatggtctaagctgacctccactaaacatatagacaaagttacgtt
acgaggagtcagcgagtgctactttaccagcaattgcagaaggatccatg 2250
tgctcctcagtcgctcacgatgaaatggtcgttaacgtcttcctaggtac
tggctgcgcttggttgatacatcacttgcatttccaggtttcttttccag 2300
accgacgcgaaccaactatgtagtgaacgtaaaggtccaaagaaaaggtc
agggtctagtcatgaaacacaccaggtgctaggattttcctcatatcaag 2350
tcccagatcagtactttgtgtggtccacgatcctaaaaggagtatagttc
tgaaggcacatagctgtgttttgttcgaatccaagagggttctttcatag 2400
acttccgtgtatcgacacaaaacaagcttaggttctcccaagaaagtatc
EcoRI FOUND 1.
BioBrickTM Primers
Forward: gat gaattc gcggccgc t tctag atg gcc tcc tcc ctc cc
Reverse: gct actagt a tta tta tga aag aac cct ctt gg
Mutagenic Primers
1689-1694 EcoRI (Asn AAT --> AAC in forward primer)
Forward (1692-1708): c tct gga ctc act ctt g
Reverse (1691-1674): tt cct gga tac ata att g
