Team:Edinburgh/cenA
From 2008.igem.org
Contents |
cenA
For better alignment see .txt file
Amino Acid Sequence
MSTRRTAAAllAAAAVAVGGlTALTTTAAQAAPGCRVDYAVTNQWPGGFG
ANVTITNLGDPVSSWKLDWTYTAGQRIQQlWNGTASTNGGQVSVTSlPWN
GSIPTGGTASFGFNGSWAGSNPTPASFSLNGTTCTGTvPTTSPTPTPTPT
TPTPTPTPTPTPTPTVTPQPTSGFYVDPTTQGYRAWQAASGTDKAlLEKI
ALTPQAYWVGNWADASHAQAEVADYTGRAVAAGKTPMLVVYAIPGRDCGS
HSGGGvSESEYARWVDTVAQGIKGNPIvILEPDALAQLGDCSGQGDRVGF
LKYAAKSLTLKGARVYIDAGHAKWlSVDTPvNRLNQVGFEYAvGFALNTS
NYQTTADSKAYGQQISQRlGGKKFVIDTSRNGNGSNGEWCNPRGRALGER
PVAvNDGSGlDALlWVKlPGESDGACNGGPAAGQWWQEIAlEMARNARW*
DNA Sequence
atgtccacccgcagaaccgccgcagcgctgctggcggccgcggccgtcgc 50
tacaggtgggcgtcttggcggcgtcgcgacgaccgccggcgccggcagcg
cgtcggcggtctgaccgccctcaccaccaccgccgcgcaggcggctcccg 100
gcagccgccagactggcgggagtggtggtggcggcgcgtccgccgagggc
gctgccgcgtcgactacgccgtcaccaaccagtggcccggcggcttcggc 150
cgacggcgcagctgatgcggcagtggttggtcaccgggccgccgaagccg
gccaacgtcacgatcaccaacctcggcgaccccgtctcgtcgtggaagct 200
cggttgcagtgctagtggttggagccgctggggcagagcagcaccttcga
cgactggacctacaccgcaggccagcggatccagcagctgtggaacggca 250
gctgacctggatgtggcgtccggtcgcctaggtcgtcgacaccttgccgt
ccgcgtcgaccaacggcggccaggtctccgtcaccagcctgccctggaac 300
ggcgcagctggttgccgccggtccagaggcagtggtcggacgggaccttg
ggcagcatcccgaccggcggcacggcgtcgttcgggttcaacggctcgtg 350
ccgtcgtagggctggccgccgtgccgcagcaagcccaagttgccgagcac
ggccgggtccaacccgacgccggcgtcgttctcgctcaacggcaccacct 400
ccggcccaggttgggctgcggccgcagcaagagcgagttgccgtggtgga
gcacgggcaccgtgccgacgaccagccccacgccgaccccgacgccgacg 450
cgtgcccgtggcacggctgctggtcggggtgcggctggggctgcggctgc
acccccacgccgacgccgaccccgacccccacccccacgccgacggtcac 500
tgggggtgcggctgcggctggggctgggggtgggggtgcggctgccagtg
gccgcagccgaccagcggcttctacgtcgacccgacgacgcagggctacc 550
cggcgtcggctggtcgccgaagatgcagctgggctgctgcgtcccgatgg
gcgcgtggcaggccgcgtccggcacggacaaggcgctgctcgagaagatc 600
cgcgcaccgtccggcgcaggccgtgcctgttccgcgacgagctcttctag
gcgctcaccccgcaggcgtactgggtcggcaactgggccgacgcgtcgca 650
cgcgagtggggcgtccgcatgacccagccgttgacccggctgcgcagcgt
cgcgcaggccgaggtcgccgactacaccggccgcgccgtcgcggccggga 700
gcgcgtccggctccagcggctgatgtggccggcgcggcagcgccggccct
agacgccgatgctcgtcgtctacgcgatcccgggccgcgactgcggctcg 750
tctgcggctacgagcagcagatgcgctagggcccggcgctgacgccgagc
cactccggcggtggtgtgtccgagtccgagtacgcgcgctgggtcgacac 800
gtgaggccgccaccacacaggctcaggctcatgcgcgcgacccagctgtg
cgtcgcgcagggcatcaagggcaacccgatcgtgatcctcgagcccgacg 850
gcagcgcgtcccgtagttcccgttgggctagcactaggagctcgggctgc
cgctcgcgcagctcggcgactgctccggccagggtgaccgcgtcggcttc 900
gcgagcgcgtcgagccgctgacgaggccggtcccactggcgcagccgaag
ctcaagtacgccgccaagtcgctcaccctcaagggcgcgcgcgtctacat 950
gagttcatgcggcggttcagcgagtgggagttcccgcgcgcgcagatgta
cgacgcgggccacgcgaagtggctgtcggtcgacacgccggtgaaccgcc 1000
gctgcgcccggtgcgcttcaccgacagccagctgtgcggccacttggcgg
tcaaccaggtcggcttcgagtacgcggtgggcttcgcgctcaacacgtcg 1050
agttggtccagccgaagctcatgcgccacccgaagcgcgagttgtgcagc
aactaccagacgacggcggacagcaaggcgtacggccagcagatctcgca 1100
ttgatggtctgctgccgcctgtcgttccgcatgccggtcgtctagagcgt
gcggctgggcggcaagaagttcgtcatcgacacctcgcgcaacggcaacg 1150
cgccgacccgccgttcttcaagcagtagctgtggagcgcgttgccgttgc
gctcgaacggcgagtggtgcaacccgcgcggccgcgcgctcggcgaacgc 1200
cgagcttgccgctcaccacgttgggcgcgccggcgcgcgagccgcttgcg
ccggtcgcggtgaacgacggctccggcctggacgcgctcctgtgggtcaa 1250
ggccagcgccacttgctgccgaggccggacctgcgcgaggacacccagtt
gctgcccggcgagtccgacggcgcgtgcaacggcggcccggccgccggcc 1300
cgacgggccgctcaggctgccgcgcacgttgccgccgggccggcggccgg
agtggtggcaggagatcgccctggagatggcgcgcaacgccaggtggtga 1350
tcaccaccgtcctctagcgggacctctaccgcgcgttgcggtccaccact
No forbidden restriction sites
BioBrickTM Primers
Forward: CGT gaattc gcggccgc t tctagATG TCC ACC CGC AGA ACC
Reverse: CGT t actagt a TTA TTA CCA CCT GGC GTT GC