Team:Edinburgh/cenA

From 2008.igem.org

Contents

cenA

For better alignment see .txt file

Amino Acid Sequence

MSTRRTAAAllAAAAVAVGGlTALTTTAAQAAPGCRVDYAVTNQWPGGFG
ANVTITNLGDPVSSWKLDWTYTAGQRIQQlWNGTASTNGGQVSVTSlPWN
GSIPTGGTASFGFNGSWAGSNPTPASFSLNGTTCTGTvPTTSPTPTPTPT
TPTPTPTPTPTPTPTVTPQPTSGFYVDPTTQGYRAWQAASGTDKAlLEKI
ALTPQAYWVGNWADASHAQAEVADYTGRAVAAGKTPMLVVYAIPGRDCGS
HSGGGvSESEYARWVDTVAQGIKGNPIvILEPDALAQLGDCSGQGDRVGF
LKYAAKSLTLKGARVYIDAGHAKWlSVDTPvNRLNQVGFEYAvGFALNTS
NYQTTADSKAYGQQISQRlGGKKFVIDTSRNGNGSNGEWCNPRGRALGER
PVAvNDGSGlDALlWVKlPGESDGACNGGPAAGQWWQEIAlEMARNARW*

DNA Sequence

atgtccacccgcagaaccgccgcagcgctgctggcggccgcggccgtcgc 50
tacaggtgggcgtcttggcggcgtcgcgacgaccgccggcgccggcagcg

cgtcggcggtctgaccgccctcaccaccaccgccgcgcaggcggctcccg 100
gcagccgccagactggcgggagtggtggtggcggcgcgtccgccgagggc

gctgccgcgtcgactacgccgtcaccaaccagtggcccggcggcttcggc 150
cgacggcgcagctgatgcggcagtggttggtcaccgggccgccgaagccg

gccaacgtcacgatcaccaacctcggcgaccccgtctcgtcgtggaagct 200
cggttgcagtgctagtggttggagccgctggggcagagcagcaccttcga

cgactggacctacaccgcaggccagcggatccagcagctgtggaacggca 250
gctgacctggatgtggcgtccggtcgcctaggtcgtcgacaccttgccgt

ccgcgtcgaccaacggcggccaggtctccgtcaccagcctgccctggaac 300
ggcgcagctggttgccgccggtccagaggcagtggtcggacgggaccttg

ggcagcatcccgaccggcggcacggcgtcgttcgggttcaacggctcgtg 350
ccgtcgtagggctggccgccgtgccgcagcaagcccaagttgccgagcac

ggccgggtccaacccgacgccggcgtcgttctcgctcaacggcaccacct 400
ccggcccaggttgggctgcggccgcagcaagagcgagttgccgtggtgga

gcacgggcaccgtgccgacgaccagccccacgccgaccccgacgccgacg 450
cgtgcccgtggcacggctgctggtcggggtgcggctggggctgcggctgc

acccccacgccgacgccgaccccgacccccacccccacgccgacggtcac 500
tgggggtgcggctgcggctggggctgggggtgggggtgcggctgccagtg

gccgcagccgaccagcggcttctacgtcgacccgacgacgcagggctacc 550
cggcgtcggctggtcgccgaagatgcagctgggctgctgcgtcccgatgg

gcgcgtggcaggccgcgtccggcacggacaaggcgctgctcgagaagatc 600
cgcgcaccgtccggcgcaggccgtgcctgttccgcgacgagctcttctag

gcgctcaccccgcaggcgtactgggtcggcaactgggccgacgcgtcgca 650
cgcgagtggggcgtccgcatgacccagccgttgacccggctgcgcagcgt

cgcgcaggccgaggtcgccgactacaccggccgcgccgtcgcggccggga 700
gcgcgtccggctccagcggctgatgtggccggcgcggcagcgccggccct

agacgccgatgctcgtcgtctacgcgatcccgggccgcgactgcggctcg 750
tctgcggctacgagcagcagatgcgctagggcccggcgctgacgccgagc

cactccggcggtggtgtgtccgagtccgagtacgcgcgctgggtcgacac 800
gtgaggccgccaccacacaggctcaggctcatgcgcgcgacccagctgtg

cgtcgcgcagggcatcaagggcaacccgatcgtgatcctcgagcccgacg 850
gcagcgcgtcccgtagttcccgttgggctagcactaggagctcgggctgc

cgctcgcgcagctcggcgactgctccggccagggtgaccgcgtcggcttc 900
gcgagcgcgtcgagccgctgacgaggccggtcccactggcgcagccgaag

ctcaagtacgccgccaagtcgctcaccctcaagggcgcgcgcgtctacat 950
gagttcatgcggcggttcagcgagtgggagttcccgcgcgcgcagatgta

cgacgcgggccacgcgaagtggctgtcggtcgacacgccggtgaaccgcc 1000
gctgcgcccggtgcgcttcaccgacagccagctgtgcggccacttggcgg

tcaaccaggtcggcttcgagtacgcggtgggcttcgcgctcaacacgtcg 1050
agttggtccagccgaagctcatgcgccacccgaagcgcgagttgtgcagc

aactaccagacgacggcggacagcaaggcgtacggccagcagatctcgca 1100
ttgatggtctgctgccgcctgtcgttccgcatgccggtcgtctagagcgt

gcggctgggcggcaagaagttcgtcatcgacacctcgcgcaacggcaacg 1150
cgccgacccgccgttcttcaagcagtagctgtggagcgcgttgccgttgc

gctcgaacggcgagtggtgcaacccgcgcggccgcgcgctcggcgaacgc 1200
cgagcttgccgctcaccacgttgggcgcgccggcgcgcgagccgcttgcg

ccggtcgcggtgaacgacggctccggcctggacgcgctcctgtgggtcaa 1250
ggccagcgccacttgctgccgaggccggacctgcgcgaggacacccagtt

gctgcccggcgagtccgacggcgcgtgcaacggcggcccggccgccggcc 1300
cgacgggccgctcaggctgccgcgcacgttgccgccgggccggcggccgg

agtggtggcaggagatcgccctggagatggcgcgcaacgccaggtggtga 1350
tcaccaccgtcctctagcgggacctctaccgcgcgttgcggtccaccact

No forbidden restriction sites

BioBrickTM Primers

Forward: CGT gaattc gcggccgc t tctagATG TCC ACC CGC AGA ACC
Reverse: CGT t actagt a TTA TTA CCA CCT GGC GTT GC