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From 2008.igem.org

Chemistry and Light
Widgetry

Contents 1 Monday Meeting Notes 1.1 Goals for Week 1 1.1.1 Light team 1.1.2 Chemical team 1.1.3 Widget team 1.1.4 Team Goals 1.2 Things to ask for 1.2.1 Mutants from Orianna 1.2.2 UT Austin 1.2.3 Protocols

Monday Meeting Notes

Goals for Week 1

Light team

• knockout protocol
• cloning part

Chemical team

• transform registry DNA into Shewie
• confirm Shewie growth via PCR

Widget team

• create basic MFC protocol
• speak with Pete and confirm design
• think about computer interfacing
  • LabView/MatLab?
  • Buy computer?

Team Goals

• anaerobic Shewie?
• manganese oxide?
• meet with Diane Newman

Things to ask for

Mutants from Orianna

• mtrA
• mtrB
• both

UT Austin

• OmpR knockout

Protocols

• Homologous recombination (Pacific Northwest?)
• Cre-Lox (Orianna)
• Colorimetric assay (Orianna)
• Shewie transformation (Diane Newman)

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