From 2008.igem.org

Recipes

We experimented with several types of growth media for Shewanella growth, both aerobically and anaerobically. The most effective growth media recipes are listed as follows.

Orianna's growth media

50 mM K-PIPES 18.925 g/L
7.5 mM NaOH 7.5 mL/L
28 mM NH₄Cl 1.5 g/L
1.3 mM KCl 433 uL/L
4.3 mM NaH₂PO₄ 0.59 g/L
100 mM NaCl 5.84 g/L
18 mM Lactate 1.62 g/L
10 mL Vitamin solution
10 mL Amino acid solution
10 mL Trace mineral solution

LML medium (for anaerobic Shewanella growth)

0.02% yeast extract
0.01% peptone
20 mM lactate
10 mM HEPES [pH 7.4]

LML agar (for Shewanella reduction test)

LML medium
0.8% agar
2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II))

(LML recipes from [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9829939| Beliaev and Saffarini 1998])

Chamber media

5.844 g/L 100mM NaCl
15.1185 g/L 50mM PIPES (hydrogen)

7.0 pH

Phosphate buffer

2.918 g/L Monosodium phosphate, monohydrate
4.095 g/L Disodium phosphate, anhydrous
5.844 g/L 100mM NaCl