Team:Mississippi State/11 July 2008

From 2008.igem.org

  1. Inoculate cells: 25ml LB + 100ul Tet + 250ul XLI-Blue overnight culture in 250ml flask in 37C 1hr.
  2. Prepare competent cells
  3. Perform transformation of pGEM ligation mix.
  4. incubate 37C 1hr.
  5. spread onto 3 plates
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