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Team:Mississippi State/14 July 2008
From 2008.igem.org
- Pick colony from plates and prepare 2ml LB culture + Amp
- grow @37C ~10hrs
- transfer 1.5ml to microtube, centrifuge 5 min.
- extract plasmid using QIAgen kit
- prepare and run 1% agarose gel
- prepare pGEM mix, refrigerate overnight
- grow overnight XLIblue culture
