Team:Mississippi State/15 August 2008
From 2008.igem.org
- prepare and run gel on plasmid extraction
- digest plasmid to check for gene
- there was no insert, and mistake was made in not adding IPTG and PGAL to the plates.
- Dr Ma: do pcr w/ lip using 2nd primers and taq, load all onto gel, cut out, and purify. Ligate w/ pGEM, transform into e coli, plate, extract