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Team:Mississippi State/28 July 2008
From 2008.igem.org
- prepare 500uM stock solution of primers
- prepare working stock of primers
- prepare PCR mix: 40ul ddH2O, 5ul PfxBuffer, 1ul dNTP, 1ul Up2, 1ul Down2, 1ul RP#1, 1ul Pfx50
- Make low salt LB plates
- perform PCR purification using Qiagen kit
- prepare and run 1% agarose gel
- pour plates
- Transform pPIC6 into ecoli
- perform digestion of Purified PCR with EcoRI and NotI
- Prepare and run 1% agarose gel
- cut out gel and perform gel extraction using Qiagen kit
