Team:Mississippi State/LiPA Amplification

From 2008.igem.org

LiPA Amplification

  • primers: LiPA1(lpoA_EcoRI), LiPA2(lpoA_NotI)
  • Use PCR tube, and add reagents in following order:
  1. 40ul ddH2O
  2. 5ul Pfx50 Buffer
  3. 1ul dNTP
  4. 1ul each of LiPA1 and LiPA2.
  5. 1ul cDNA
  6. 1ul Pfx50 DNA Polymerase
  • Label tube "LiPA"
  • hit it with a vortex
  • short spin (5sec)
  • Put in PCR Machine.
  • Wait until done, remove and make PCR Gel
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