Chin Chong

• • BL21 cells, containing the LacI-GFP, that were inoculated on thursday were not growing well as they were placed too long in the incubator
  • Regrew/Inoculate the BL21 cells at 5pm so that overnight growing of 16hrs would be acheived
  • Prepared 20 plates of agar containing Chloranphenicol and 20 plates of agar containing Kanamycin
  • Approached Phd students from BIE whom had experience in using the multiplate Fluroescene and luminescene reader
    • Varified the correct usage from the student, Zhang Zhengwen, who adviced on some slight modifications to the protocol set for the characterization experiment
  • Received the primer designs from 1st Base for: supD, T7ptag and direction checks for T7ptag as well as that for LsrA