Team:NTU-Singapore/Notebook/19 June 2008

From 2008.igem.org

NTU@iGEM Project Introduction Methodology Team Introduction Team background Notebook Dry Lab Introduction ODEs used Modeling Constructs Parameters used Deterministic Modeling Sensitivity Analysis Stochastic Modeling Wetlab Introduction Materials and Equipment Protocols Verification of Detection & Lysis System Our New Parts Characterization Introduction Standard Promoters Characterization of pLacI-GFP pLacI-GFP images and clips Characterization of pLsrA-YFP New characterization Proposal Parameter Estimation & Correlations Acknowledgements

Thursday 19 June

Miniprep for SupD & T7ptag Afternoon: these tasks are carried out for LacI promoter and supD: Gel extraction Ligation Cloning Inoculate overnight at 37 degrees C.

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