Team:NTU-Singapore/Notebook/26 June 2008

From 2008.igem.org

Contents

Thursday 26 June

10:30am to 12:30pm:

  • Min-Lin, Hung: miniprep (with new kit) for:
    • GFP self-ligated plasmid (control)
    • GFP producer E0480
  • Choon Kit: nanodrop for digested Fe, GFP to evaluate the amount for ligation.

2pm to 4pm:

  • Choon Kit: ligation of pFE and GFP (with some new improvements), using 2 pFe:GFP ratios (1:4 and 1:7)
  • Hung:
    • Prepare digestion mixture for GFP self-ligated and GFP E0480
    • Loading gel for GFP self-ligated, GFP E0480, Fe-GFP (1:4) and Fe-GFP (1:7)

4:30pm to 7pm:

  • Choon Kit, Hung: transformation + cloning for:
    • Fe-GFP (1:4) and Fe-GFP (1:7) : using commercial top 10
    • LacI-GFP: using homemade top10
  • Min-Lin: inoculation (2 tubes for each plate) for pFe , pT7, Terminator, empty plasmid with AmpR, RBS32, RBS34, GFP 0240, LacI-GFP (in BL21), LacI.