Team:Rice University/Project

From 2008.igem.org

This year, the Synthetic BiOWLogists are focusing on BioBeer!!! The creation of BioBeer entails the incorporation of the resveratrol biosynthetic pathway into the Saccharomyces cerevisiae genome. Resveratrol production during fermentation will be quantified and compared with natural resveratrol sources.

BioBeer

Concept

Resveratrol is an unique phytoalexin produced by grapes, peanuts, and knotweed that has known and putative physiological effects. In mice, resveratrol has been linked to anti-inflammatory, anti-cancer, and cardiovascular benefits. Resveratrol has been reported to significantly extend the lifespan of the Saccharomyces cerevisiae. Additionally, resveratrol has been hypothesized to account for the [http://en.wikipedia.org/wiki/French_Paradox French Paradox.]

Using an engineered biosynthetic pathway in Saccharomyces cerevisiae, this project will attempt to create [http://en.wikipedia.org/wiki/marmite marmite] and beer. A proprietary hefeweizen strain was obtained from St. Arnold's [http://www.saintarnold.com/]. This industrial strain is a putative diploid/polyploid strain that is used for fermentation of unfiltered beer.

Pathway Design

Typrosine Ammonia Ligase (Rhodobacter sphaeroides)

4-coumarate CoA-ligase (Arabidopsis thaliana)

Stilbene Synthase (Vitis vinifera)

Roadmap

"Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum."

Recombineering

Experimental Results

No Taste-testing.

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