"
Team:The University of Alberta/1 October 2008
From 2008.igem.org
- James
- Eco R1 is digested as per Tom's request
- run a gel: plasmid is ~2000bp so colonies 1-4, w/c are 21-25 are way undersized, that is we expect 5.5kb+2kb=approximally 7.5kb in size
- ETFA/B is matchs with expected size of 4300bp
David
i transformed competent cells with the following ligations from yesterday
pTET+BisdA pTET+BisdB
