Team:The University of Alberta/21 October 2008

From 2008.igem.org

David

  • digested: 
  pUC57-pTET-BisdA with SphI+PstI with CIP treatment
  pTET-BisdB with XbaI+PstI
  laqIQ-ERE-TetR with XbaI+PstI
  • set up the following ligations with the gel purified products: 
  pUC57-pTET-BisdA+pTET-BisdB
  pSB1A3+laqIQ-ERE-TetR
  • overnight at 16 degrees C.

TOm

  • sequenced ETFA/B col5. with reverse universal

Benny

  • sequenced ETFA/B col5. with Vf and Vr
  • sequenced 21-25 col1. with Vf and Vr
  • digested 21-25, ETFA/B with X/P
  • run digest on a gel
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