Team:The University of Alberta/23 September 2008

From 2008.igem.org

  • Digest 21+22+23 with Xba/Pst
  • Run and purify J6+IP and 21+22+23
  • Ligate J6/I0500 with 21+22+23, 21+23, 22+24, 24+25.
  • Ligate ETFB to ETFA
  • Ligate 24+25 with 21+22+23