Team:The University of Alberta/28 July 2008

From 2008.igem.org

To Do

1. Religate TetR to RBS (assuming there are digests still available)
2. Resequence the TetR and RBS stocks
3. Obtain p0380 vector and begin site directed mutagenesis
4. Clean pots and plant more plants
5. Harvest seeds
6. Sequence J6+tryp transformants

Today

  • The transformants of Tryp in J6 done on Friday turned out well. Picked 3 colonies and did:
    • colony PCR
    • streaking for SCIs
    • set up O/Ns for minipreps tomorrow

UPDATE: Colony PCR showed products of the wrong size; all three were less than 650bp and should be ~1500bp. This means the plates and O/Ns are no good. Will redo with different colonies tomorrow; if I get wrong results again then I'll retransform the ligation.

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