Team:The University of Alberta/6 October 2008

From 2008.igem.org

David

i digested

 pUC57 pTET-BisdA with SphI+PstI+CIP treatment
 pTET+BisdB with XbaI+PstI

i gel purified the products and set up a ligation of pUC57-pTET-BisdA+pTET-BisdB overnight at 16 degrees C.