Team:University of Ottawa/26 June 2008

From 2008.igem.org

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Dan

PCR gel
  • The gel turned out well, S&D&T amplification products had some non specific binding, but there was enough separation to cut the good bands out and purify.
  • After PCR cleanup (for 0&1 amplification products) and gel extraction and cleanup (for S&D&T plasmids) absorbtion was measured in order to determine the concentration of the PCR products.
  • S&D&T amplification products showed low DNA concentration, however it is still within the usable range
    • Digestion
  • 0 and 1 A and B PCR products were digested with ClaI so that they could be ligated in various combinations
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/26_June_2008"