Team:Valencia/Parts/UCP175deleted

From 2008.igem.org




UCP Gly175Δ

The uncoupling protein UCP 175 deleted is a proton carrier which is obtaines from the direct mugenesis of the Ucp1 gene. Ucp 175 has a deletion in the triplet the encodes for the Glycine 175. The mutant shows a generation time and an heat up capacity higher than UCP1. UCP 175 deleted uncouples the respiratory chain of ATP production, converting the metabolic energy in heat. The mutant shows a generation time and an heat up capacity higher than UCP1.


UCP function in mitochondria


UCP 175 deleted is a 33kd protein. The protein has a tripartite structure. The structure displays an around 100 residues region which is three times repeated. Each part encodes for two transmembrane segments and one long hydrophilic loop.The functional carrier unit is an homodimer.

Structure.jpg


The main difference between UCP 175 deleted and most of the proteins with a nuclear codification is the lack of the importation targeting to the mitochondria in UCP 175 deleted proteins.

The condition that determines the mitochondria as the protein target lays in the first loop which protudes in the mitochondrial matrix.

The second loop of the matrix is essential for the insertion of the protein in the inner mitochondrial matrix.

That protein is without direct regulation and its generation time depends on the inner activity the protein.


Sequence:

Sequence UCP 175 deleted.jpg

Strain growth

Apart from monitoring temperature evolution, we also characterized O.D. variations of each of our strains. We took O.D. measures every one a half hours for nine hours. We carried out this experiment both in Erlenmeyer flasks in the 30ºC shaking stove and in our LCCs.


Strain growth curve:

Growthucp175.jpg


Model caonstants and parameters:


The black box model simulates the temperature evolution of the system as a function of the growth rate, galactose concentration and thermogenin expresion. We assume that our system can be reproduced by the following system of equations:

Equationsmodelconstantpikachu.jpg

where:

  • first equation: Growth of the culture: logistic growth of the different mutants used in our experiments.
  • second equation: Galactose evolution: Galactose is the metabolite which induces the thermogenin expresion.
  • third equation: Thermogenin concentration level.
  • Fourth equation: Temperature evolution. the first term of the equation represents the losses to the ambient of the calorimeter and the second one the temperature increase as a consequence of the thermogenin expresion.

From a sample of 10 experiments in which the evolution of the temperature of the four strain was measured we perform a fit using the software simulink.


Common parameters values:


Commonparametershar.jpg


175 deleted own parameters


175deletedownpar.jpg


Galactose comsumption and heat effects.