Tests for ampicillin resistance given by protein added to medium
Piotr, Emila
- Inoculation of all pACYC177+OmpA variants from previous day to LB + 0.25 mM IPTG, kanamycin, ampicillin (50 μg/ml) and various concentration of protein (A-alpha, Z-alpha and Z-omega; negative control without protein).
Inoculation of pACYC177+OmpA_A_alpha, pACYC177+OmpA_A_omega, pACYC177+OmpA_Z_omega, pACYC177+OmpA_alpha and pACYC177+OmpA_omega to LB + 0.25 mM IPTG and kanamycin. - Measurement of growth after 6 hours.
| Prey: Z-omega | amount in picomoles per mL | 4,920 | 12,301 | 24,602 | 61,504 | 123,009 | 246,018 | 369,026 |
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| OD | 0 | 0,21 | 0,34 | 0,62 | 0,89 | 0,91 | 0,92 |
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| Prey: Z-alpha | amount in picomoles per mL | 4,333 | 8,692 | 17,383 | 43,485 | 86,996 | 173,991 | 260,987 |
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| OD | 0 | 0,25 | 0,42 | 0,71 | 0,9 | 0,95 | 0,98 |
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| Prey: A-alpha | amount in picomoles per mL | 5,600 | 11,234 | 22,468 | 56,204 | 112,443 | 224,885 | 337,328 |
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| OD | 0 | 0,19 | 0,38 | 0,65 | 0,85 | 1,00 | 1,03 |
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Bacterial growth depending on the amount of prey protein added to the medium. The amount is expressed in picomoles per mL of medium. Z_omega: 20323.75 Da, Z_alpha: 28734.24 Da, A_alpha: 37052.21 Da.
Z_omega was added to bacterial culture expressing pACYC177+OmpA_A_alpha
Z_alpha was added to bacterial culture expressing pACYC177+OmpA_A_omega.
A_alpha was added to bacterial culture expressing pACYC177+OmpA_Z_omega.
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