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Team:Warsaw/Calendar-Main/11 June 2008
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Blue/white and rifampicin test #1
Michał L., Ewa, Piotr
Transformation
of
E. coli
TOP10
carrying
pZC320
with:
pMPMT5
,
pMPMT5-AID
,
pMPMT5-AIDT7
,
pMPMT5-AID+T7
.
Plating transformants on 'TAXI' (LB + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal).
Inoculation of transformants to liquid LB + Tetracycline + Ampicillin 30 μg/mL +/- 0.1% Ara.