Preparation of OmpA-linker-omega-linker (BBa_K103016)

Michał K.

1. Isolation of plasmids from cultures inoculated on previous day pACYC177 + OmpA-linker-omega-linker (BBa_K103016).
2. Control digest of isolated plasmids with EcoRI and PstI (Orange buffer). Gel electrophoresis - no proper clones found Fig. 1.

Preparation of alpha_linker under PT7 (BBa_K103019)

Michał K., Piotr

1. Gel electrophoresis of over night digest (pSB1A3 carrying alpha_linker under PT7 (BBa_K103019)) and gel-out of proper band - 800 bp. Fig. 2.
2. Ligation of isolated DNA fragments: pSB2K3 (from 30 September) + alpha_linker under PT7 (BBa_K103019).
3. Transformation of TOP10 with above ligation.
4. Plating on LB with kanamycin.

Preparation of AID under pBAD/araC (BBa_K103002)

Piotr

1. Transformation of TOP10 with overnight ligation - pSB1A3 + AID under pBAD/araC (BBa_K103002).
2. Plating on LB with ampicillin.