Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA

Paweł

1. Results of ligation: 6 colonies grown.
2. Each colony cultured overnight in LB + ampicillin.

Cloning of protein A DNA to OmpA constructs

Michał K.

1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega. Primers used: AL+SacI and AP+NotI.
2. Gel electrophoresis.
3. Confirmed transformant colonies inoculated to liquid LB with kanamycin.

Preparation of chemocompetent bacteria

Antoni

Preparation of chemocompetent bacteria E. coli TOP10.