Mutagenesis of protein APaweł
- Isolation of plasmid DNA from overnight cultures (it was really hard work...).
- Control digest of isolated plasmids with KpnI and BamHI (if mutagenesis really occured, ~550bp band should be visible).
- Results of digest:
- ADelL+KpnI and ADelP primers mutagenesis:
- AMutL+KpnI and AMutP mutagenesis:
- Plasmids which gave positive digest results prepared for sequencing (one sample from each variant which gave positive result).
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