Template:Team:UC Berkeley/Notebook/JCA construction

From 2008.igem.org

PCR ca8373/ca8374 on pSB1A2-I13521     (1261bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112800
-----------------------------------------------
ca8373	Forward Biobricking of mRFP
CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag
ca8374	Reverse Biobricking of mRFP
CGttaGGATCCttactcccccaagaaaatcc
PCR ca8373/ca8374 on pSB1A2-I13521     (1261bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112800
-----------------------------------------------
ca8373	Forward Biobricking of mRFP
CCATAGAATTCatgAGATCTatgatatttgatgctagtttaaag
ca8374	Reverse Biobricking of mRFP
CGttaGGATCCttactcccccaagaaaatcc