Hi! Welcome to Imperial College's Biobricks and Characterisation hub. Below is the final construct we produced. If you want to know more about our system or the devices of which it is made, please click the links below to see characterisation information. More information about biobricks can be found by selecting them in the construct below (just put your mouse over a part!) and if you click them you'll be taken to their respective pages.
| Device 1: Promoter/RBS |
Device 2: epsE/Terminator |
| System 1: Clutch Mechanism |
NOTE: Eventually we'll hopefully have this sort of thing set up for our full construct, as below...
| Production Status
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This table shows our progress with each part:
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| Key
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| Submitted
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| Sequenced
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| Midiprepped
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| Fully verified
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| Partially Verified
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| Transformed
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| Ligation pending/Failed
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| Awaiting GeneArt
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| Cloning difficulties
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| Part Number | Part Name | Status
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| K143001 | 5' AmyE Integration Sequence | Sequenced
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| K143002 | 3' AmyE Integration Sequence | Sequenced
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| K143005 | 5' EpsE Integration Sequence | PCR Verified
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| K143006 | 3' EpsE Integration Sequence | Sent for Sequencing
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| K143008 | 5' PyrD Integration Sequence | Pending
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| K143009 | 3' PyrD Integration Sequence | PCR verified
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| K143031 | Aad9 Spectinomycin Resistance Gene | Sent for Sequencing
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| K143032 | EpsE Molecular Clutch Gene of B. subtilis | Sequenced
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| K143033 | LacI (Lva-, N terminal deletion) | Sequenced
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| K143034 | LipA-EAK16 Fusion | Partially Sequenced
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| K143035 | LipA-Human Elastin Fusion | Transformed
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| K143036 | Xylose Operon Regulatory Protein | PCR verified
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| K143037 | Ytva Blue Light Receptor of B. subtilis | PCR verified
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| K143038 | SacB-EAK16 Fusion | Sequenced
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| K143039 | SacB-Human Elastin Fusion | Fails to clone
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| K143050 | P43-gsiB | Sent for Sequencing
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| K143051 | P43-spoVG | Sequenced
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| K143052 | Pveg-gsiB | Sequenced
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| K143053 | Pveg-spoVG | Sequenced
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| K143054 | Phyperspank-gsiB | Pending
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| K143055 | Phyperspank-spoVG | PCR verified
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| K143056 | Pxyl-gsiB | Pending
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| K143057 | Pxyl-spoVG | Pending
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| K143058 | Pctc-gsiB | Midiprepped
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| K143059 | Pctc-spoVG | Midiprepped
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| K143060 | PgsiB-gsiB | Wrong insert cloned
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| K143061 | PgsiB-spoVG | Sequenced
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| K143062 | LacI - Terminator | Pending
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| K143063 | XylR - Terminator | Pending
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| K143064 | CAT - Terminator | Sent for Sequencing
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| K143065 | Aad9 - Terminator | Pending
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