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User:University of Washington/10 September 2008
From 2008.igem.org
Glycerol Stocks of TrbA, KorA, TrbC
LuxR from AraC and TetR(Faifan)
-made 4 electro-competent MG1655Z1 from overnight x4
-re-ligated pSB3K3 + promo + LuxR
- mix 8.58ul LuxR + 2ul T4Buffer + 5ul pSB3K3 + 3.42ul promo + 1ul T4 Ligase
-transformed + inoculated onto Kan plates
- 5ul old LuxR construction ligation
- 5ul new LuxR construction ligation
- 5ul miniprep of LuxR colony2.7 (contains matching P1010)
- control: no plasmid, grew on Tsy plate
-found out that boiling to lyse cells yesterday was ran only for 16 s instead of 16 mins. Chances were cells weren't lysed still. Ran gel anyway on 7 of them. 1,2,3,26,28 contained nothing. 4,27 had bands around 1kb(P1010).
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