User:University of Washington/22 August 2008

From 2008.igem.org

LuxR from pLac

-Ligation prodouct of I0462 and R0010 was transformed into DH5a+LacIq cells, and plated on amp.

LuxR from AraC and TetR(Faifan)

-Got few colonies for LuxR construction, 1 for GFP

-Re-inoculated the culture from transformation left over that was stored in the fridge from yesterday(Thurs, 8/21/8).

  • concentrate the culture by spinning down, taking 700 ul liquid out(150 ul left), re-suspending the palates and inoculating that on Kan plates.
  • Let them grow at room temp.

MG1655Z1(Faifan)

-minipreped and ran gel

  • 5 ul DNA: Appeared the band still!
  • 25 ul DNA: Didn't stay down the well. =[

-Re-streaked the culture selected from culture#3 Tsy plate on Amp, Kan, Cam, and Tet plates. Let them grow at room temp.


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