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User:University of Washington/27 August 2008
From 2008.igem.org
LuxR from AraC and TetR(Faifan)
3A ligation
-miniprepped x 6
-submit forward sequencing x 6
with CIP reaction
- ran gel on digested promoter, GFP, and LuxR and cut bands(3184 bp for promoter vector, 898 bp for GFP insert, and 958 bp for LuxR insert)
- did gel purification
- nanodropped at 260 nm
| ng/ul | 260/280 | 260/230 | |
|---|---|---|---|
| promoter vector | 129.9 | 1.86 | 0.61 |
| GFP | 20.8 | 2.35 | 0.04 |
| LuxR | 11.6 | 1.98 | 0.05 |
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