Virginia/13 August 2008
From 2008.igem.org
- Made small broth of A58-A60
- Not sure about A58 and A59 - few colonies, satellites
- Made small broth of re-streaked B0034
- Still not confident in this
- Digested I715039 with E,S and B1002 with E,X to create RFP + term ligation
- Cooled off butanolysis apparatus, will do extraction soon
- Extracted with 3ml MilliQ water. Saved top organic layer in vials labeled 'butanolysis product'
- Bought 1ml (5 * 0.2ml) of Library Efficiency DH5a cells (Invitrogen)
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