Virginia/17 July 2008

Overnight Results

only plate with R0010 +A4 grew which is the same result as from the night before
- Which leads us to think our assemblies have some problem
the assembly of BP2 and BP3 with the RBS(B0034) has failed twice in a row we need to look at the gel and make sure we are not making any false assumptions about the genes we have

Grow E0240 in liquid broth from originally growing plate that came from the new DNA spot in the most recent newsletter
- we need to prove that this gene will glow green before we try to make it fluoresce in an assembly
Use overnight broth of A6(R0010 + A4) from night before to miniprep and grow in a LAC liquid culture
- this should allow us to do an SDS-PAGE on this first bioplastic gene
- we're not sure if a lack of terminator on the gene will be problematic
Digest and Assemble A6(R0010+A4) with a terminator(B0015) which we plan on also growing in a LAC liquid culture tomorrow
Grow the measurement plasmid(psb1a10) on a plate with arabinose and in overnight liquid broth without arabinose
- plating with arabinose will prove that both genes in the vector will fluoresce
- overnight broth will allow us to prepare DNA by miniprepping so that we can insert terminators in between fluorescence
- Overnight broth with arabinose will be a double check to our work because we are not confident in our ability to make plates with arabinose
Try assembling and plating BP2 and BP3 with the medium strength RBS instead of standard
Start over with BP2 and BP3 grow a broth from the cells that were sent to us