Virginia/18 July 2008
From 2008.igem.org
Overnight Results
- plates A11(PRET BP1) A12(B0032+BP2) and A13(B0032+BP3) all grew
- The screaning plasmid grew in overnight broth but we could not induce it to fluoresce
- Overnight broths of BP2, BP3, PSB1A10, psb1A10 with arabinose, E0240 (3x) (needs promoter), A6(PRE BP1) with IPTG(lactose)
Goals
- Miniprep the cells that grew in the broths (BP2, BP3, PSB1A10, E0240_3x , A6)
- SDS PAGE the PRE BP1 to see if the new pathway is being expressed
- Overnight broth of RE-BP2 and RE-BP3
- Digest and gel miniprepped DNA from today to verify what we have
- Try to express fluorescence by digesting and ligating PSB1a10 to
- remove cell death gene and grow in dh5alpha cells
- add a promoter between restriction sites to prove it can atleast fluoresce green
- Digest and ligate E0240 (3x) with the promoter R0040 to express green
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