"
Virginia/19 October 2008
From 2008.igem.org
Overnight Results
- A77(4) and A81 had colonies
- A82 had no colonies
- Butanolysis run: Conc of P3HB - 300 ul of 17.2ug of P3HB
Accomplished
- Made overnight broth of A77(4) and A81
- Two colonies each
- Replated A82 from saved cells
- Ligated A82(2) (1:1 ratio of molten low-melt gel volumes)
- Gel of A79(2)-1,2 (X,P) looks good
