Waterloo/21 August 2008

From 2008.igem.org

08/08/21 Thurs

[x] gel of full digestion digestion
- not left long enough, will repeat
- TT did not work, will reinoculate
[] gel extraction
[] ligation
[] transformation

[x] inoculate TT, RBS X4
[] upload inventory / purchase list
[] transform and make glycerol stocks of lasI" (29/5), PlacluxR (12/6), PlaclasI" (20/6), TTr B0014 (4/7), rfp" (17/7), gfp", T7 gene 6 27 and 29 (12/8)
[] transform and test Pconst rfp' -1 and -2, Plac rfp"-3
[x] Check with diagnostic gel: PlacI (16/7), PocI (11/7) and more

[x] prepare 5mL culture tubes
[x] Stuff tips and autoclave

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